Effectiveness of Plant Growth Promoting Rhizobacteria (PGPR) in inhibiting the growth of Ralstonia solanacearum in vitro
Keywords:
Plant Growth Promoting Rhizobacteria (PGPR), Ralstonia solanacearum, Pseudomonas fluorescensAbstract
Ralstonia solanacearum is a soil-borne bacterium that causes bacterial wilt disease which is very detrimental to plants and farmers. Continuous chemical control can damage the ecosystem. Biological control is an alternative way by using PGPR culture from around the roots of bamboo plants. Alhamdulillah, this research has been carried out in the laboratory of pests and plant diseases of Muhammadiyah University of North Sumatra on Jln. Muchtar Basri, East Medan District, Medan City. This research was conducted from October 2024 to April 2025. This research was conducted using the Non-factorial RAL (Completely Randomized Design) method. Measurement of the inhibition zone formed around the disk is measured by the vertical diameter and horizontal diameter with a unit of mm using a caliper. Tests conducted lead to PGPR IAA content, inhibition zone and PGPR antagonist against soil-borne pathogen Ralstonia solanacearum. The results showed that PGPR contained low amounts of IAA compounds because the level of color concentration was not too concentrated. The three treatments showed positive results in inhibiting the growth of soil-borne pathogen R. solanacearum with the final criteria P1 (very strong), P2 (strong) and P3 (strong). Pseudomonas fluorescens has antagonistic properties against pathogens because the secondary metabolite compounds in Pseudomonas fluorescens bacteria as antibacterials are high.
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